Ayako is from a family of martial arts and she is the older sister of Minori Mitsuzuri. Ayako is a second year student in Homurahara Academy. She is a stellar athlete, and captain of the archery club that Taiga Fujimura advises. Ayako and Rin Tohsaka actively compete to see who will be the first to snag a boyfriend. Although she wants to find a boyfriend, it seems like she wants to stay single as long as possible.
Ayako forms a team with her classmates Kane Himuro and Ayaka Sajyou competed in the Homurahara Academy's ball games tournaments in a match of sepak takrawWP. They faced Shirou Emiya and his classmates Issei Ryuudou (who managed to gets both teams disqualified) and Shinji Matou.
She has a strong-looking appearance, but privately she's actually pretty girly. Of course, this clashes with her image so she doesn't go around advertising it, but she doesn't really think it's a big deal herself.
She is friends with both Shirou and Rin. While she treats Rin as her friend, she treats Shirou more like a rival. The three of them haven't noticed what a delicate relationship they have with each other. Even though her room at home looks more like it belongs to a little girl than a teenager, she has no qualms about inviting Rin over to hang out. After all, it's not that she has no interest in romance, it's just that she has a thing for older men. She holds the belief of a heroine that "Beauty must do martial arts".
In Fate/stay night, she is first seen in the prologue when Rin encounters her in the morning. She was practicing her archery for the first year. Ayako often asks Shirou to come to the archery dojo and watch them practice. She informs Shirou about Shinji bullying the male first year students when he was in a foul mood when Rin rejected him.
She was seen arguing with Shinji Matou in the Archery Dojo before her disappearance. She was later found unconscious in an alleyway in Shinto. She was victimized by Rider when Rider chased her down into a back alley and sucked her blood on the spot, in order to extract magical energy. When Shirou asked if Shinji was involved, Shinji denies it.
In Fate/hollow ataraxia, she first appears in the prologue telling a ghost story about Person A. She is in her third year and has already retired from the captain position of the archery club. She's very uncomfortable around Rider, who shows a disconcerting attraction to her like an animal fond of its prey.
Creation and Conception
Her character design is based on Rider's earlier concept. During the preliminary stages of development, the scenario director was obsessed with a movie called "Vi(…)ck", and kept on insisting that Rider should be more like a certain black-caped character. Ayako's design was an attempt to meet his demands that unfortunately ended up being rejected. Nasu says that it is forbidden to say she resembles Shiki Ryougi.
- In the first Popularity polls of Fate/stay night in 2004, Ayako was ranked 11th.
- In the second Popularity polls of Fate/stay night in 2006, Ayako was ranked 17th.
- In the Type-Moon's 10th Anniversary Character Poll, Ayako was ranked 60th.
 Fate/complete material II: Character Material - Characters: Ayako Mitsuduri, p.108-109
Ayako Mitsuduri - The Archery club captain from a family of the martial arts
Character Data 020
Affiliation: Homurahara Academy, 2 Year class A
Three sizes: B83/W58/H83
Image Color: Orange
Talents: Martial arts in general
Likes: Video games in general
Dislike: Go, Shogi
Worst enemy: Taiga Fujimura
Nasu Kinoko & Takeuchi Takashi • Character Discussion
>The catalyst for Ayako Mitsuzuri's birth
Nasu: Ayako is another character we created to flesh out the school aspect of the story. As with Rin, writing Ayako's character felt really good. She's an honourable and manly sort, but not violent. Here's a bit of trivia: early on in the story, when it's still unclear as to who the masters are, I was going to use Ayako and Issei as red herrings just for fun. The conversation between Ayako and Rin near the beginning of the game can be quite misleading, but it's really just a bit of healthy competition between friends. I felt like Rin needed a good female friend, so even though Ayako is a totally minor character in the story, I put a lot of thought into her.
>Ayako and Rin
Ayako and Rin have a friendly competition going on to see who can get a boyfriend first, but Ayako is more of a dreamer and has some pretty high standards when it comes to potential boyfriends. Unfortunately, Mr Right has yet to come along. Most of the boys at her school like her well enough but view her as more of a big sister, and their exaggerated respect sometimes gets on her nerves.
>Ayako and Shirou
Takeuchi: I feel like the relationship between Ayako and Shirou is a little... vague.
Nasu: Shirou is not one to recognise or show off his own skills, but Ayako sees that he is naturally gifted at archery. It is truly a shame for someone like Ayako that he does not seem to recognise his own talent... so I guess she feels a blend of admiration and disappointment toward Shirou.
Nasu: Born into a well-respected family in the martial arts world. Ayako is learned in most forms of martial arts but is a modern girl on the inside. She hides this side of herself in public in order to keep up appearances for her family, but she is a total gamer. Her family is quite strict about anything that is not related to martial arts, so Ayako is forced to play her video games secretly in her room. Ayako plays video games because she enjoys them, and practices martial arts out of a sense of duty. That is not to say she hates martial arts or anything, as she would not have become as skilled as she is if she didn't enjoy it on some level. The whole "strict family" thing may be something she has in common with Kaede Makidera, but compared to Ayako, Kaede is a total free spirit. (laughs)
>Ayako and the Holy Grail War
Nasu: Aside from that encounter with Rider, Ayako didn't really get caught up in the Holy Grail War much at all. If this game was made by a certain other company, I imagine the scene between Rider and Ayako would have been a lot more erotic... Talk about missed opportunities! (laughs)
美綴綾子 - 武道の家系出身の弓道部主将
Character Data ０２０
奈須 きのこ&武内崇 • キャラクター別対談
奈須：美綴も学園パートのキャラクターです ね。彼女に関しては、凛と同じように書いてて気持ちのいい子にしました。１本筋が入つた男前だけど乱暴じゃない。今だから語つてしまう と、誰がマスターか分からないつていう序盤の流れの中で、美綴も一成もその撒き餌ではあったんです。本当に思わせぷりですが。ゲーム序盤の凛との会話もミスリードを誘つていますけど、ただの女の子同士の競い合いです。凛にも女の友人が必要だと思いましたので。チョイ役ではあるんですけど、熱を入れて書いたキャラクターのひとりです。
奈須：士郎にはてらいがなく、そして美綴が武芸の中で唯一苦手とする弓道において「あ、ち ょっとコイツ神懸かってる」と感じた男ですか ら。ただ士郎自身はそのことを意識していな い。だから彼女は「ちょっともったいないな」 って。
奈須：彼女は聖杯戦争には、ほとんど巻き込まれていません。ライダーにちょい噛まれたぐら いかな？ これが某メー力一だったら、ライダーに工口工口な行為をされちゃうんですけど ね（笑）。残念でした！
 Fate/side material - Encyclopedia: Mitsuduri Ayako [Person's name], p.075-076 [T]
Ayako Mitsuduri [Person's name]
A female student in Class 2-A.
She is the captain of the archery club, and friends with both Emiya Shirou and Tohsaka Rin.
Well, Rin is her friend, while she treats Shirou more like a rival. The three of them haven't noticed what a delicate relationship they have with each other.
Her big-sisterly disposition exceeds even Rin's, and she's considered one of the top three students at Homurahara Academy to avoid picking a fight with.
People tend to think that she's mannish, but privately she's actually pretty girly.
Of course, this clashes with her image so she doesn't go around advertising it, but she doesn't really think it's a big deal herself.
Even though her room at home looks more like it belongs to a little girl than a teenager, she has no qualms about inviting Rin over to hang out. After all, it's not that she has no interest in romance, it's just that she has a thing for older men. Could it be? She and Rin are united by their father complex!?
Though it may seem odd that a supporting character has such spirited looks, it's only natural given that originally her design was conceived of for Rider.
During the preliminary stages of development, the scenario director was obsessed with a movie called "Vi(…)ck", and kept on insisting that Rider should be more like a certain black-caped character.
Mitsuzuri's design was an attempt to meet his demands that unfortunately ended up being rejected.
Still, it seemed like a waste to just throw out such good work, so it was ultimately resurrected and recycled into Mitsuzuri Ayako. Thank goodness, yeah?
Saying she resembles Ryougi Shiki is strictly prohibited.
By the way, after the Rin route she's still as energetic as ever. And in case you were wondering, yes, the person who attacked her was Rider. She chased her down into a back alley and SLUUURP, sucked her blood on the spot! I sure hope she didn't pick up any weird habits from that, nyahaha.
 Fate/complete material III: World material - FAQ with Nasu: Characters, p.134-135
Q: Will Ayako ever have a boyfriend?
Q: Will Ayako ever have a boyfriend?
A: It seems like she wants to find one, but it also seems like she wants to stay single as long as possible... Miss Mitsuzuri is still fully interested in maidenhood, so she’ll probably be shot down soon after she enters college. By a young man suited to black-rimmed glassses, harmless to man and beast. ...I think I’ve seen a photo of those two somewhere...
Ａ：できてほしいような、できる限りひとり身でいてほしいような……。美綴嬢はあれで乙女趣 味全開なので、大学に進学した先であっさり撃墜されるかもしれません。黒縁眼鏡の似合う、 人畜無害そうな青年に。……あれ、そのツーショットどっかで見たぞ……？
- Fate/strange Fake - Volume 2 - Interlude: "The Little Red Riding Hood of Semina Apartments", p.013-021