Major Matou is identical to his mainline counterpart Shinji Matou.
Major Matou summons Berserker with the intent of summoning Miyamoto Musashi, but instead summons Mori Nagayoshi, who was known as the Demon Musashi. Berserker almost immediately turns on Matou, cutting off his arm so that Command Spells could not be used against him. He proceeded to carry Major Matou with him as he cut a path through the Imperial Japanese forces. Matou was eventually rescued by Caren Kotomine and Lancer.